ABSTRACT
Fifty-seven Thai herbs and spices were examined for their retroviral reverse transcriptase inhibitory activity. All herbs and spices were extracted with hot-water and methanol. Reverse transcriptase inhibitory activity of the extracts was determined by using Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV-RT) reacted with 3H-dTTP and radioactivity measured with a scintillation counter. Eighty-one per cent (46/57) of hot-water extracts and 54% (31/57) of methanol extracts showed inhibitory activities. At a concentration of 125 micrograms/ml, 13% (6/46) of hot-water extracts, namely Eugenia caryophyllus Bullock et Harrison, Phyllanthus urinaria Linn., Terminalia belerica Roxb., Nelumbo nucifera Gaertn., Psidium guajava Linn. and Lawsonia inermis Linn., had a relative inhibitory ratio (IR) over 50%. They showed ratios of 100%, 91%, 75%, 74%, 61% and 60%, respectively. For methanol extracts, only 10% (3/31) had IR values over 50%. They were T. belerica, E. caryophyllus and N. nucifera which exhibited IR values of 83%, 54% and 54%, respectively.
Subject(s)
Animals , Enzyme Inhibitors/isolation & purification , Leukemia Virus, Murine/enzymology , Mice , Plants, Medicinal/chemistry , Retroviridae/enzymology , Reverse Transcriptase Inhibitors , Spices/analysis , ThailandABSTRACT
O objetivo da presente revisäo é mostrar alguns aspectos relacionados com o reverso da transcriçäo, incluindo descriçäo do retrovírus, ciclo de vida do vírus, mecanismo do reverso da transcriçäo, propriedades cinéticas e imunológicas da transcritase reversa, e inibidores da enzima viral. Estäo também incluídas algumas referências sobre o retrovírus AIDS. O reverso da transcriçäo parece näo ser restrito apenas aos vírus de RNA. Alguns vírus de DNA, como o vírus da hepatite B, e o vírus do mosaico da couve-flor, e outros elementos genéticos, como pseudogenes e elementos de transposiçäo, parecem utilizar o reverso da transcriçäo para suas existências